This study was designed to investigate the relationship between Type 2 diabetes mellitus (T2DM) and musculoskeletal system disorders and kinesiophobia levels. Eighty-four participants (41 diabetics and 43 nondiabetic individuals) self-reported their musculoskeletal problems and levels of kinesiophobia. Data were analyzed using descriptive statistics, nonparametric Pearson’s Chi-Square testing, and Mann–Whitney U test. Results indicated the total Cornell Musculoskeletal Discomfort Questionnaire score was 190.55 ± 261.56 for the patients with T2DM and 98.11 ± 167.31 for the control group (p < 0.05). Moreover, Tampa Kinesiophobia Scale scores were higher for the T2DM group (p < 0.05). We conclude from these findings that T2DM is associated with a higher frequency of musculoskeletal system disorders and higher levels of kinesiophobia.